Estimation of miRNA-21, miRNA-422, miRNA‐142-3p, and miR-181c Gene Expression as Potential Biomarkers of Osteoporosis in Premenopausal Women

Nahidah kzar madhloom; Maan Hasan Salih; Iktefa Abdul Hamid Mohammed saeed; Shimaa Jumaa Abood

doi:10.26505/djm.v28i2.1295

PDF

Published Jun 25, 2025

DOI https://doi.org/10.26505/djm.v28i2.1295

Nahidah kzar madhloom

Department of Biology, College of Education for women, Tikrit University, Tikrit, Iraq.

Maan Hasan Salih

Department of Biology, College of Sciences, Tikrit University, Tikrit, Iraq.

Iktefa Abdul Hamid Mohammed saeed

Department of Biology, College of Education for women, Tikrit University, Tikrit, Iraq.

Shimaa Jumaa Abood

Department of Biology, College of Education for Pure Sciences, Tikrit University, Tikrit, Iraq.

Abstract

Background: MicroRNAs (miRNAs) regulate several biological pathways in osteoporosis patients. The study aimed to estimate the correlation of miRNA-21, miRNA-422, miRNA‐142-3p, and miR-181c with osteoporosis in premenopausal women.

Patients and Methods: This study was performed on 60 osteoporosis premenopausal and 30 healthy women. Determination of osteocalcin (OC), deoxypyridinoline (DPD), and bone-specific alkaline phosphatase (BAP) were done by ELISA, and estimation of miRNA gene expression was done by using qRT-PCR.

Results: OC, DPD, and BAP levels indicated a significant decrease in osteoporosis women. In addition, our data showed that miRNA-21 (p=0.0001, r=-0.5585) and miRNA-422 (p=0.0035, r=-0.3715) have a high expression and negative correlation with BMD. Meanwhile, the miRNA-142-3p (p=0.0136, r=0.3089) and miRNA-181c (p=0.0401, r=-0.2685) have decreased expression and positive correlation with BMD value.

Conclusion: The results of this study indicated a clear association of miRNA-21 and miRNA-422 in osteoporosis thus they may be useful as biomarkers for osteoporosis.

Keywords: Osteoporosis, miRNA-21, miRNA-422, miRNA‐142-3p, and miR-181c.

Downloads

Download data is not yet available.

How to Cite

[1]

Nahidah kzar madhloom, Maan Hasan Salih, I. A. H. M. saeed, and Shimaa Jumaa Abood, “Estimation of miRNA-21, miRNA-422, miRNA‐142-3p, and miR-181c Gene Expression as Potential Biomarkers of Osteoporosis in Premenopausal Women”, djm, vol. 28, no. 2, pp. 40–52, Jun. 2025.