Isolation and Purification of metalloprotease produced by Aeromonas hydrophila

Abstract

Background: The bacteria Aeromonas hydrophila was isolated from clinical specimens responsible of causing diarrhea in children and adults.

Objective:To purify metalloprotease enzyme produced by Aeromonas hydrophila.

Patients and Methods: This study involved 150 stool samples collected from patients suffering from diarrhea.

Results: Eight isolate of Aeromonas hydrophila was detected and metalloprotease was purified with 11.06 U/ml of enzyme activity.

Conclusion: The bacteria have ability to produce metalloprotease and enzyme was fully purified in three steps of purification.